LLC-MK2, original

Origin: rhesus, monkey, kidney.

Anat. Rec. 1956. 124: 490; Natl.Cancer Inst. Monograph. 1962. 7: 161;

J.Exp.Med. 1962. 115: 903; J.Gen.Virol. 1979. 43: 289.

Morphology: epithelial-like

Mode of cultivation: monolayer

Conditions for cultivation: medium - EMEM

serum -   FBS  10%

subculture procedure - cells detach from flask using EDTA 0.02%

with 0.1 mg/ml chimopsin, split ratio 1:3-1:5, optimal population density 1.0105 cells/ml

cryoconservation - EMEM 70%, FBS 20%, glycerol 10%, 3.0-5.0106 cells/ml in ampule

Viability after cryoconservation: 92% (0 passage, dye trypan blue)

Sterility: tests for bacteria, fungi and mycoplasma were negative

Species: karyological and isoenzymological (LDH, G6PD) analysis

Karyology:   2n= 42, variability in the range between 56-69 chromosomes,

modal number of chromosomes 68, number of markers - 1 large submetacentric

chromosome with a gap (routine dye), number of polyploid cells 16.0%.

Other properties:

virus susceptibility: arboviruses; enteroviruses; vesicular stomatitis, polioviruses 1-3,

parainfluenza 1-3, influenza A, B; rhinoviruses, myxoviruses, poxviruses.

Applications: virology.

Collections: ATCC CCL 7; ECACC 85062804; MWIIW.