LLC-MK2, original (ESCC)
rhesus, monkey, kidney, subline of LLC-MK2, original.
Anat. Rec. 1956. 124: 490.
Mode of cultivation:
Conditions for cultivation:
medium - 199/BME (1:1)
serum - BS 5-7%
subculture procedure - cells detach from flask using
EDTA 0.02%, split ratio 1:4-1:5, optimal population density 0.8-1.0õ105 cells/ml
cryoconservation - growth medium, glycerol 10%, 1.0-1.5õ106 cells/ml in ampule
Viability after cryoconservation:
80-85% (0 passage, dye trypan blue)
tests for bacteria, fungi and mycoplasma were negative
karyological and isoenzymological (LDH, G6PD) analysis
Karyology: 2n= 42, variability in the range between 44-64 chromosomes,
modal number of chromosomes 56-60.
virus susceptibility: arboviruses; enteroviruses; adenoviruses.