LLC-MK2, original (ESCC)

Origin: rhesus, monkey, kidney, subline of LLC-MK2, original.

Anat. Rec. 1956. 124: 490.

Morphology: epithelial-like

Mode of cultivation: monolayer

Conditions for cultivation: medium - 199/BME (1:1)

serum -   BS 5-7%

subculture procedure - cells detach from flask using

EDTA 0.02%, split ratio 1:4-1:5, optimal population density 0.8-1.0105 cells/ml

cryoconservation - growth medium, glycerol 10%, 1.0-1.5106 cells/ml in ampule

Viability after cryoconservation: 80-85% (0 passage, dye trypan blue)

Sterility: tests for bacteria, fungi and mycoplasma were negative

Species: karyological and isoenzymological (LDH, G6PD) analysis

Karyology:   2n= 42, variability in the range between 44-64 chromosomes,

modal number of chromosomes 56-60.

Plating efficiency:  80 %

Other properties:

virus susceptibility: arboviruses; enteroviruses; adenoviruses.

Applications: virology.

Collections: ESCC.