LLC-MK2, derivative

Origin: rhesus monkey, kidney, derived from LLC-MK2 original.

            Anat.Res. 1956. 124: 490; J.Gen.Virol. 1979. 43: 289.

Morphology:  epithelial-like

Mode of cultivation: monolayer

Conditions for cultivation: medium - DMEM or EMEM

serum -   FBS or BS 10%

other components -   NEAA 1% (EMEM)

subculture procedure - cells detach from flask using trypsin 0.25%:

EDTA  0.02% (1:3), split ratio 1:3, optimal population density 2.0-4.0104  cells/cm2

cryoconservation - growth medium (may add 30% BS), 5-7%DMSO, 1.0-1.5106 cells/ml in ampule

Viability after cryoconservation:     80-90% (0 passage, dye trypan blue)

Sterility: tests for bacteria, fungi and mycoplasma were negative

Species: karyological and isoenzymological (LDH, G6PD and nucleoside phosphorylase) analysis

Karyology:   2n= 42, variability in the range between 63-73 chromosomes,

modal number of chromosomes 67-70, number of markers - 1-4 middle submetacentrics

with the second constriction, number of polyploid cells 4.8%.

Plating efficiency:    45% (ATCC)

Other properties:

virus susceptibility: poliovirus 1, 2, 3, parainfluenza 2, 3

Applications:  virology.

Collections: ATCC CCL 7.1; SPBIC; SPBII.