mouse C3H, subcutaneous adipose connective tissue, clone of NCTC clone 929.
J.Natl.Cancer Inst. 1948. 9: 228
Mode of cultivation:
Conditions for cultivation:
serum - BS 10%
subculture procedure - cells detach from flask using EDTA 0.02%,
split ratio 1:3-1:5, optimal population density 1.0-1.3x105 cells/ml
cryoconservation - growth medium, 20% glycerol, 1.5-2.0õ106 cells/ml in ampule
Viability after cryoconservation:
90% (0 passage, dye trypan blue)
tests for bacteria, fungi and mycoplasma were negative
karyological and isoenzymological (LDH, G6PD) analysis
Karyology: 2n= 40, variability in the range between 36-67 chromosomes,
modal number of chromosomes 54.
virus susceptibility: arboviruses, togaviruses.
Presence of oncoviruses A and C.