Lk

Origin: mouse C3H, subcutaneous adipose connective tissue, clone of NCTC clone 929.

            J.Natl.Cancer Inst. 1948. 9: 228

Morphology: epithelial-like

Mode of cultivation: monolayer

Conditions for cultivation: medium - 199/EMEM (1:1)

serum -   BS 10%

subculture procedure - cells detach from flask using EDTA 0.02%,

split ratio 1:3-1:5, optimal population density 1.0-1.3x105 cells/ml

cryoconservation - growth medium, 20% glycerol, 1.5-2.0106 cells/ml in ampule

Viability after cryoconservation:   90% (0 passage, dye trypan blue)

Sterility: tests for bacteria, fungi and mycoplasma were negative

Species: karyological and isoenzymological (LDH, G6PD) analysis

Karyology:   2n= 40, variability in the range between 36-67 chromosomes,

modal number of chromosomes 54.

Plating efficiency:    80%

Other properties:

virus susceptibility: arboviruses, togaviruses.

Presence of oncoviruses A and C.

Applications: virology.

Collections: ESCC