LES

Origin: pig, embryo, lung.

            Inf.Bull. Cell Culture Ass. St.-Petersburg, 1997, N12.

Morphology: epithelial-like and fibroblast-like

Mode of cultivation: monolayer

Conditions for cultivation: medium - DMEM/LAH (1:1)

serum -   BS 10%

subculture procedure - cells detach from flask using trypsin 0.25%:

EDTA  0.02% (1:9), split ratio 1:2-1:3.

cryoconservation - culture medium 50%, BS 40%, DMSO 10%, 2.0-3.0106 cells/ml in ampule

Viability after cryoconservation:     79% (0 passage, dye trypan blue)

Sterility: tests for bacteria, fungi and mycoplasma were negative

Species: karyological analysis

Applications: cell biology, virology.

Collections: MWIEV