LEK VIEV-90 ref

Origin: cattle, embryo, lung.

RF Patent N17781885, 1.08.92; Zamaraeva H.B., PhD thesis, M., 1993.

Morphology: fibroblast-like

Mode of cultivation: monolayer

Conditions for cultivation: medium - 0.25% EMPH-d

serum -  BS 10%

subculture procedure - cells detach from flask using trypsin 0.25%:

EDTA  0.02% (1:9), split ratio 1:5.

cryoconservation - culture medium 50%, BS 40%, DMSO 10%, 2.0-3.0106 cells/ml in ampule

Viability after cryoconservation:     75% (0 passage, dye trypan blue)

Sterility: tests for bacteria, fungi and mycoplasma were negative

Species: karyological analysis

Other properties:

production of BLV.

Applications: biotechnology.

Collections: MWIEV