LEK

Origin: cattle, embryo, lung.

J. Veterinary ( Russ). 1985. 10: 35-37.

Morphology: epithelial-like

Mode of cultivation: monolayer

Conditions for cultivation: medium -  BME or MWPH or EMPH-d

serum -   BS 10%

subculture procedure - cells detach from flask using trypsin 0.25%:

EDTA  0.02% (1:9), split ratio 1:2-1:3.  

cryoconservation - culture medium 45%, BS 45%,  DMSO 10%, 3.5-4.0106 cells/ml in ampule

Viability after cryoconservation: 80 % (0 passage, dye trypan blue)

Sterility: tests for bacteria, fungi and mycoplasma were negative

Species: karyological  analysis

Karyology:   2n=60, modal number of chromosomes 58-60.

Other properties:

virus susceptibility: equine rhinopneumonia; calf rotavirus; parainfluenza 3;

bovine infectious rhinotracheitis.

Applications: virology , biotechnology

Collections: MWIEV