mouse C3H, malignated subcutaneous adipose connective tissue, clone of NCTC cl.929.
Submitted by Tomsk RIVS from MRIVP, 1975.
Mode of cultivation:
Conditions for cultivation:
serum - BS 10%
subculture procedure - cells detach from flask using EDTA 0.02%,
split ratio 1:4 -1:5, optimal population density 1.0 -1.3x105 cells/ml
cryoconservation - growth medium, 10% glycerol, 1.0 -1.5 õ106 cells/ml in ampule
Viability after cryoconservation:
80% (0 passage, dye trypan blue)
tests for bacteria, fungi and mycoplasma were negative
karyological and isoenzymological (LDH, G6PD) analysis
2n=40, variability in the range between 35-56 chromosomes, modal number of chromosomes 52
virus susceptibility: arboviruses; arenaviruses.
Presence of A and C oncoviruses and paramyxoviral ribonucleoproteid.