Origin: mouse C3H, malignated subcutaneous adipose connective tissue, clone of NCTC cl.929.

Submitted by Tomsk RIVS from MRIVP, 1975.

Morphology:  fibroblast-like

Mode of cultivation: monolayer

Conditions for cultivation: medium - 199/EMEM (1:1)

serum -   BS 10%

subculture procedure - cells detach from flask using EDTA 0.02%,

split ratio 1:4 -1:5, optimal population density 1.0 -1.3x105 cells/ml

cryoconservation - growth medium, 10% glycerol, 1.0 -1.5 106 cells/ml in ampule

Viability after cryoconservation: 80% (0 passage, dye trypan blue)

Sterility: tests for bacteria, fungi and mycoplasma were negative

Species: karyological and isoenzymological (LDH, G6PD) analysis

Karyology:   2n=40, variability in the range between 35-56 chromosomes, modal number of chromosomes 52 

Plating efficiency:   80 %

Other properties:

virus susceptibility: arboviruses; arenaviruses.

Presence of A and C oncoviruses and paramyxoviral ribonucleoproteid.

Applications: virology.

Collections: ESCC