L 1210

Origin: mouse DBA/2, lymphocytic leukemia, ascitic fluid.

            J.Natl.Cancer Inst. 1966. 36: 405-421.

Morphology:  lymphoblast-like

Mode of cultivation: suspension

Conditions for cultivation: medium - RPMI 1640

serum -   FBS 10%

subculture procedure - optimal population density 5.0104 - 8.0105 cells/ml

cryoconservation - growth medium, 10% DMSO, 2.0106 cells/ml in ampule

Viability after cryoconservation:     90% (0 passage, dye trypan blue)

Sterility: tests for bacteria, fungi and mycoplasma were negative

Species: karyological analysis

Karyology:  2n= 40, variability in the range between 34-42 chromosomes,

modal number of chromosomes 39-41, number of polyploid cells 0.2%.

Tumorigenicity:  tumorigenic in singeneic and nude mice

Applications:  cytotoxicity, tumorigenicity, cell biology.

Collections: ATCC CCL 219; ECACC 87092804; SPBIC.