mouse DBA/2, lymphocytic leukemia, ascitic fluid.
J.Natl.Cancer Inst. 1966. 36: 405-421.
Mode of cultivation:
Conditions for cultivation:
medium - RPMI 1640
serum - FBS 10%
optimal population density
5.0õ104 - 8.0õ105 cells/ml
cryoconservation - growth medium, 10% DMSO,
2.0õ106 cells/ml in ampule
Viability after cryoconservation:
90% (0 passage, dye trypan blue)
tests for bacteria, fungi and mycoplasma were negative
Karyology: 2n= 40, variability in the range between 34-42 chromosomes,
modal number of chromosomes 39-41, number of polyploid cells 0.2%.
tumorigenic in singeneic and nude mice
tumorigenicity, cell biology.
ATCC CCL 219; ECACC 87092804; SPBIC.