muscle cells transformed by methylcholanthrene, derived from L6.
Exp.Cell Res. 1979. 120: 1; Cytology (Russ). 1983, 25: 1096-1097..
Mode of cultivation:
Conditions for cultivation:
medium - DMEM
serum - FBS 10%
subculture procedure - cells detach from flask using trypsin 0.25%:
EDTA 0.02% (1:3), split ratio 1:10 - 1:15, do not allow cultures
to become completely confluent.
cryoconservation - growth medium, 10% DMSO,
1.0x106 cells/ml in ampule
Viability after cryoconservation:
85% (0 passage, dye trypan blue)
tests for bacteria, fungi and mycoplasma were negative
karyological, isoenzymological (LDH, G6PD) and immunofluorescent analysis
Karyology: 2n= 42, variability in the range between 41-47 chromosomes,
modal number of chromosomes 42-43,number of markers- 3 (differential dye)
some cells have one small submetacentric chromosome with gap in short arm
microchromosoma (routine dye), number of polyploid cells 5%.
differentiates producing myotubes, synthesis of muscle specific proteins.