L6J1

Origin: rat, skeletal muscle cells transformed by methylcholanthrene, derived from L6.

            Exp.Cell Res. 1979. 120: 1; Cytology (Russ). 1983, 25: 1096-1097..

Morphology:  myoblast-like

Mode of cultivation: monolayer

Conditions for cultivation: medium - DMEM

serum -   FBS 10%

subculture procedure - cells detach from flask using trypsin 0.25%:

EDTA  0.02% (1:3), split ratio 1:10 - 1:15, do not allow cultures

to become completely confluent.

cryoconservation - growth medium, 10% DMSO, 1.0x106 cells/ml in ampule

Viability after cryoconservation:     85% (0 passage, dye trypan blue)

Sterility: tests for bacteria, fungi and mycoplasma were negative

Species: karyological, isoenzymological (LDH, G6PD) and immunofluorescent analysis

Karyology:   2n= 42, variability in the range between 41-47 chromosomes,

modal number of chromosomes 42-43,number of markers- 3 (differential dye)

some cells have one small submetacentric chromosome with gap in short arm

microchromosoma (routine dye), number of polyploid cells 5%.

Plating efficiency:    42%

Other properties:

differentiates producing myotubes, synthesis of muscle specific proteins.

Applications:  differentiation, myogenesis.

Collections:  SPBIC.