L-M (TK-, APRT-)
mouse, connective, derived from NCTC clone 929.
Submitted Institute of Biochemistry, Martinsried, FGR.
Mode of cultivation:
Conditions for cultivation:
medium - DMEM
serum - FBS 10%
subculture procedure - cells detach from flask using trypsin 0.25%:
EDTA 0.02% (1:3), split ratio 1:5 - 1:10, optimal population density 2.0-3.0õ104 cells/cm2
cryoconservation - growth medium, 10% FBS, 5-10% DMSO, 1.8õ106 cells/ml in ampule
Viability after cryoconservation:
68% (0 passage, dye trypan blue)
tests for bacteria, fungi and mycoplasma were negative
karyological, isoenzymological (LDH, G6PD) and immunological analysis
Karyology: 2n= 40, variability in the range between 46-51 chromosomes,
modal number of chromosomes 49, number of markers - 9 metacentrics (routine dye).
deficient in thymidine kinase and adenine phosphoribosyl
transferase (resistant to 5-bromodeoxyuridine and 8-azaadenine.
Retrovirus type A production
virology, somatic cell genetics, cell biology.