L-8

Origin: rat Wistar, skeletal muscle.

            Develop.Biol. 1970. 23: 1-22; Differentiation 1977. 7: 159-166.

Morphology:  myoblast-like

Mode of cultivation: monolayer

Conditions for cultivation: medium - DMEM or EMEM

serum -   FBS 10%

subculture procedure - cells detach from flask using trypsin 0.25%:

EDTA  0.02% (1:3), split ratio 1:10

cryoconservation - growth medium, 10% DMSO, 1.0x106 cells/ml in ampule

Viability after cryoconservation:     90% (0 passage, dye trypan blue)

Sterility: tests for bacteria, fungi and mycoplasma were negative

Species: karyological and isoenzymological (LDH, G6PD) analysis

Karyology:   2n=42, variability in the range between 36-42 chromosomes,

modal number of chromosomes 39, some cells have 1-2 large acrocentric

chromosomes (routine dye), number of poliploid cells 1.0%.

O ther properties:

synthesise several specific proteins characteristic of muscle tissue.

Differentiates forming multinucleated muscle fiber.

Applications:  differentiation, cell biology.

Collections: ATCC CRL 1769; SPBIC.