rat Wistar, skeletal muscle.
Develop.Biol. 1970. 23: 1-22; Differentiation 1977. 7: 159-166.
Mode of cultivation:
Conditions for cultivation:
medium - DMEM or EMEM
serum - FBS 10%
subculture procedure - cells detach from flask using trypsin 0.25%:
EDTA 0.02% (1:3), split ratio 1:10
cryoconservation - growth medium, 10% DMSO, 1.0x106 cells/ml in ampule
Viability after cryoconservation:
90% (0 passage, dye trypan blue)
tests for bacteria, fungi and mycoplasma were negative
and isoenzymological (LDH, G6PD)
Karyology: 2n=42, variability in the range between 36-42 chromosomes,
modal number of chromosomes 39, some cells have 1-2 large acrocentric
chromosomes (routine dye), number of poliploid cells 1.0%.
synthesise several specific proteins characteristic of muscle tissue.
Differentiates forming multinucleated muscle fiber.
differentiation, cell biology.
ATCC CRL 1769; SPBIC.