KTP

Origin: pig, testis.

            USSR Patent N 1389282, 1985.

Morphology: epithelial-like

Mode of cultivation: monolayer

Conditions for cultivation: medium - 0.5% hemohydrolizate or BME/LAH (1:1)

serum -   BS 10%

subculture procedure - cells detach from flask using trypsin 0.25%:

EDTA  0.02% (1:), split ratio 1:5

cryoconservation - culture medium 50%, BS 40%, DMSO 10%,

2.0-3.0106 cells/ml in ampule

Viability after cryoconservation:     80% (0 passage, dye trypan blue)

Sterility: tests for bacteria, fungi and mycoplasma were negative

Species: karyological analysis

Other properties:

virus susceptibility: swine corona- and enteroviruses.

Applications: virology, biotechnology.

Collections: MWIEV