KST

Origin: cattle, embryo, heart coronary vessels.

            Cytology (Russ.) 1992. 34, N 9. USSR Patent N 1835849, 1992.

Morphology: epithelial-like

Mode of cultivation: monolayer

Conditions for cultivation: medium - BME

serum -   BS 7-10%

subculture procedure - cells detach from flask using trypsin 0.25%:

EDTA  0.02% (1:9), split ratio 1:3-1:4

cryoconservation - BME 45%, BS 45%, DMSO10%,

 2.0-3.0106 cells/ml in ampule

Viability after cryoconservation:    80-90 % (0 passage, dye trypan blue)

Sterility: tests for bacteria, fungi and mycoplasma were negative

Species: karyological analysis

Karyology:   2n=60, variability in the range between 58-61 chromosomes,

 modal number of chromosomes 58-60.

Tumorigenicity:  tumorigenic in nude mice

Other properties:

virus susceptibility: diarrhea, infectious rhynotracheitis of cattle.

Applications: cell biology, virology.

Collections: MWIEV