KShS

Origin: pig, thyroid.

            USSR Patent N 1394717, 1985.

Morphology: epithelial-like

Mode of cultivation: monolayer

Conditions for cultivation: medium - 0.5% hemohydrolizate

serum -   BS 10%

subculture procedure - cells detach from flask using trypsin 0.25%:

 EDTA  0.02% (1:9), split ratio  1:3-1:5

cryoconservation - culture medium 50%, BS 40%, DMSO 10%,  

2.0-3.0106 cells/ml in ampule

Viability after cryoconservation:     80% (0 passage, dye trypan blue)

Sterility: tests for bacteria, fungi were negative

Species: karyological analysis

Karyology:   2n= 38, modal number of chromosomes 34.

Other properties:

virus susceptibility: swine entero-, corona-, rotaviruses.

Applications: virology, biotechnology.

Collections: MWIEV