Submitted by Kolesnicova G.G. et al., EVIRI, 1992
Mode of cultivation:
Conditions for cultivation:
serum - BS 10%
subculture procedure - cells detach from flask using EDTA 0.02%,
split ratio 1:4-1:5, optimal population density 0.8 -1.0x105 cells/ml
cryoconservation - growth medium, 10% glycerol, 1.0õ106 cells/ml in ampule
Viability after cryoconservation:
85% (0 passage, dye trypan blue)
tests for bacteria, fungi and mycoplasma were negative
karyological and isoenzymological (LDH, G6PD) analysis
Karyology: 2n= 44, variability in the range between 42-50 chromosomes,
modal number of chromosomes 44.
virus susceptibility: herpes simplex; enteroviruses; parainfluenza 3.