Origin: rabbit, intestine.

Submitted by Kolesnicova G.G. et al., EVIRI, 1992

Morphology:  fibroblast-like

Mode of cultivation: monolayer

Conditions for cultivation: medium - 199/EMEM (1:1)

serum -  BS 10%

subculture procedure - cells detach from flask using EDTA 0.02%,

split ratio 1:4-1:5, optimal population density 0.8 -1.0x105 cells/ml

cryoconservation - growth medium, 10% glycerol, 1.0106 cells/ml in ampule

Viability after cryoconservation: 85% (0 passage, dye trypan blue)

Sterility: tests for bacteria, fungi and mycoplasma were negative

Species: karyological and isoenzymological (LDH, G6PD) analysis

Karyology:   2n= 44, variability in the range between 42-50 chromosomes,

modal number of chromosomes 44.

Plating efficiency:   70 %

Other properties:

virus susceptibility: herpes simplex; enteroviruses; parainfluenza 3.

Applications: virology, biotechnology.

Collections: ESCC