KEO

Origin: sheep, embryo, skin.

VIEV Bull. 1992. 7: 108-112 Moscow.

Morphology: epithelial-like  and fibroblast-like

Mode of cultivation: monolayer

Conditions for cultivation: medium -  199 or BME

serum -   FBS 10%

subculture procedure - cells detach from flask using trypsin 0.25%:

 EDTA  0.02% (1:9), split ratio 1:2

cryoconservation - 199 or BME 50%, FBS 40% DMSO 10%,

2.0-3.0106 cells/ml in ampule

Viability after cryoconservation: 72% (0 passage, dye trypan blue)

Sterility: tests for bacteria, fungi and mycoplasma were negative

Species: karyological analysis

Karyology:   2n= 54, diploid.

Other properties:

virus susceptibility: ovine contagious ecthyma.

Applications: virology, cell biology , biotechnology 

Collections: MWIEV