sheep, embryo, skin.
VIEV Bull. 1992. 7: 108-112 Moscow.
epithelial-like and fibroblast-like
Mode of cultivation:
Conditions for cultivation:
medium - 199 or BME
serum - FBS 10%
subculture procedure - cells detach from flask using trypsin 0.25%:
EDTA 0.02% (1:9), split ratio 1:2
cryoconservation - 199 or BME 50%, FBS 40% DMSO 10%,
2.0-3.0õ106 cells/ml in ampule
Viability after cryoconservation:
72% (0 passage, dye trypan blue)
tests for bacteria, fungi and mycoplasma were negative
2n= 54, diploid.
virus susceptibility: ovine contagious ecthyma.
virology, cell biology , biotechnology