rabbit, embryo, skin.
Cytology (Russ.) 1992. 34: 102. Rysmendeeva K.K., PhD thesis, Moscow, 1992.
epithelial-like and fibroblast-like
Mode of cultivation:
Conditions for cultivation:
medium - EMEM
serum - BS 10%
subculture procedure - cells detach from flask using trypsin 0.25%:
EDTA 0.02% (1:9), split ratio 1:2-1:3
cryoconservation - EMEM 50%, BS 40%,
DMSO 10%, 2.0-3.0õ106 cells/ml in ampule
Viability after cryoconservation:
75% (0 passage, dye trypan blue)
tests for bacteria, fungi and mycoplasma were negative
2n= 44, diploid.
virus susceptibility: ovine contagious ecthyma.
cell biology, virology, biotechnology.