KEK

Origin: rabbit, embryo, skin.

            Cytology (Russ.) 1992. 34: 102. Rysmendeeva K.K., PhD thesis, Moscow, 1992.

Morphology: epithelial-like and fibroblast-like

Mode of cultivation: monolayer

Conditions for cultivation: medium - EMEM

serum -   BS 10%

subculture procedure - cells detach from flask using trypsin 0.25%:

EDTA  0.02% (1:9), split ratio 1:2-1:3

cryoconservation - EMEM 50%, BS 40%, DMSO 10%, 2.0-3.0106 cells/ml in ampule

Viability after cryoconservation:     75% (0 passage, dye trypan blue)

Sterility: tests for bacteria, fungi and mycoplasma were negative

Species: karyological analysis

Karyology:   2n= 44, diploid.

Other properties:

virus susceptibility: ovine contagious ecthyma.

Applications: cell biology, virology, biotechnology.

Collections: MWIEV