KEK-92

Origin: rabbit, embryo, intestine.

Submitted by Shmeleva N.A. et al, EVIRI, 1986

Morphology: fibroblast-like

Mode of cultivation: monolayer

Conditions for cultivation: medium - 199/EMEM (1:1)

serum -   BS 10%

subculture procedure - cells detach from flask using EDTA 0.02%,

split ratio 1:3 optimal population density 1.0x105 cells/ml

cryoconservation - growth medium, 10% glycerol, 2.0 106 cells/ml in ampule

Viability after cryoconservation:   80-85% (0 passage, dye trypan blue)

Sterility: tests for bacteria, fungi and mycoplasma were negative

Species: karyological and isoenzymological (LDH, G6PD) analysis

Karyology:   2n=46, variability in the range between 40-60 chromosomes,

modal number of chromosomes 44 

Plating efficiency:  65 %

Other properties:

virus susceptibility: herpes simplex 1,2; CMV; enteroviruses; parainfluenza 3.

Applications: virology, biotechnology.

Collections: ESCC