rat, fibroblasts spontaneously transformed in vitro.
Submitted from N.K.Belisheva, Institute of Cytology of the USSR Academy of Sciences,
Dissert. work, 1979. L.
Mode of cultivation:
Conditions for cultivation:
medium - BME
serum - BS 10%
subculture procedure - cells detach from flask using trypsin 0.25%:
EDTA 0.02% (1:3), split ratio 1:10
cryoconservation - growth medium, 5-10% DMSO, 1.0x106 cells/ml in ampule
Viability after cryoconservation:
80% (0 passage, dye trypan blue)
tests for bacteria, fungi and mycoplasma were negative
and isoenzymological (LDH, G6PD)
Karyology 2n= 42, variability in the range between 41-44 chromosomes,
modal number of chromosomes 42, 15% of cells have 78-83 chromosomes.