K-22

Origin: rat, fibroblasts spontaneously transformed in vitro.

            Submitted from N.K.Belisheva, Institute of Cytology of the USSR Academy of Sciences,

Leningrad, 1976. Dissert. work, 1979. L.

Morphology:  fibroblast-like

Mode of cultivation: monolayer

Conditions for cultivation: medium - BME

serum -   BS 10%

subculture procedure - cells detach from flask using trypsin 0.25%:

EDTA  0.02% (1:3), split ratio 1:10

cryoconservation - growth medium,  5-10% DMSO, 1.0x106 cells/ml in ampule

Viability after cryoconservation:     80% (0 passage, dye trypan blue)

Sterility: tests for bacteria, fungi and mycoplasma were negative

Species: karyological and isoenzymological (LDH, G6PD) analysis

Karyology   2n= 42, variability in the range between 41-44 chromosomes,

modal number of chromosomes 42, 15% of cells have 78-83 chromosomes.

Tumorigenicity:  highly tumorigenic

Applications:  cell biology.

Collections: SPBIC