rat, sarcoma, derived from cell line Jensen Sarcoma.
Cancer Res. 1959. 19: 591; Cell 1975. 6: 53-60.
Mode of cultivation:
Conditions for cultivation:
medium - DMEM
serum - FBS 5-10%
other components -
- cells detach from flask using
EDTA 0.02%, split ratio 1:10.
cryoconservation - growth medium, 10% DMSO,
1.0x106 cells/ml in ampule
Viability after cryoconservation:
79% (0 passage, dye trypan blue)
tests for bacteria, fungi and mycoplasma were negative
karyological and isoenzymological (LDH, G6PD) analysis
Karyology: 2n= 42, variability in the range between 49-61 chromosomes,
modal number of chromosomes 52-56, number of markers - 1
middle acrocentric chromosome with gap (routine dye).
requires asparagine for growth
somatic cell genetics, tumorigenicity.