JF 1

Origin: rat, sarcoma, derived from cell line Jensen Sarcoma.

            Cancer Res. 1959. 19: 591; Cell 1975. 6: 53-60.

Morphology:  fibroblast-like

Mode of cultivation: monolayer

Conditions for cultivation: medium - DMEM

serum -   FBS 5-10%

other components -   NEAA 1%

subculture procedure - cells detach from flask using EDTA 0.02%, split ratio 1:10.

cryoconservation - growth medium,  10% DMSO, 1.0x106 cells/ml in ampule

Viability after cryoconservation:     79% (0 passage, dye trypan blue)

Sterility: tests for bacteria, fungi and mycoplasma were negative

Species: karyological and isoenzymological (LDH, G6PD) analysis

Karyology:   2n= 42, variability in the range between 49-61 chromosomes,

modal number of chromosomes 52-56, number of markers - 1

middle acrocentric chromosome with gap (routine dye).

Plating efficiency:    46%

Tumorigenicity:  highly tumorigenic

Other properties:

requires asparagine for growth

Applications:  somatic cell genetics, tumorigenicity.

Collections: SPBIC