J -774

Origin:  mouse BALB/c, histiocytic sarcoma.

            J.Biol.Chem. 1987. 262: 8884; J.Cell Biol. 1988. 106: 657; Proc.Natl.Acad.Sci. 1984. 81: 5430.

Morphology:  star- and round-shaped

Mode of cultivation: monolayer

Conditions for cultivation: medium - DMEM

serum -  FBS 10%

subculture procedure - cells detach from flask using trypsin 0.25%:

EDTA  0.02% (1:3), split ratio 1:3 - 1:5

cryoconservation - growth medium,  10% DMSO, 1.0x106 cells/ml in ampule

Viability after cryoconservation:     60% (0 passage, dye trypan blue)

Sterility: tests for bacteria, fungi and mycoplasma were negative

Species: karyological analysis

Tumorigenicity:  tumorigenic in syngeneic animals

Other properties:

phagocytosis, chemotaxis, antigen presentation.

Applications:  immunology, cytotoxicity, cell biology.

Collections: SPBIC.