Indian Muntjac

Origin: muntjac, skin.

Science 1970.168: 1364-1366; Cytogenet.Cell Genet.1979.24: 201-208

Morphology:  fibroblast-like

Mode of cultivation: monolayer

Conditions for cultivation: medium - F10 or F12

serum -   FBS 20%

subculture procedure - cells detach from flask using trypsin 0.25%:

EDTA  0.02% (1:3), split ratio 1:2.

cryoconservation - growth medium, 8-10% DMSO, 1.0-1.5106 cells/ml in ampule

Viability after cryoconservation:   95% (0 passage, dye trypan blue)

Sterility: tests for bacteria, fungi and mycoplasma were negative

Species: karyological and isoenzymological (LDH and G6PD) analysis

Karyology:   2n= 7, variability in the range between 5-12 chromosomes,

modal number of chromosomes 7, normal Muntjac karyotype (7, X,Y1,Y2),

number of polyploid cells 3%.

Plating efficiency: 29% (ATCC)

Other properties:

virus susceptibility: vesicular stomatitis, herpes simplex, vaccinia.

Applications:  genetics, morphology, virology, cell biology.

Collections: ATCC CCL 157; MWIIW; SPBIC.