HTC

Origin: rat Buffalo, hepatoma induced by N,N-2,7-fluorenylenebis-2,2,2-trifluoroacetamide, ascitic fluid.

            Proc.Natl.Acad.Sci. 1966. 56: 296; ATLA 1988. 16: 32.

Morphology:  epithelial-like

Mode of cultivation: monolayer

Conditions for cultivation: medium - DMEM or EMEM

serum -   FBS 10%

other components -   NEAA 1% (EMEM)

subculture procedure - cells detach from flask using trypsin 0.25%:

EDTA  0.02% (1:1), split ratio 1:5

cryoconservation - growth medium,  10% DMSO, 1.0106 cells/ml in ampule

Viability after cryoconservation:    90% (0 passage, dye trypan blue)

Sterility: tests for bacteria, fungi and mycoplasma were negative

Species: karyological and isoenzymological (LDH, G6PD) analysis

Karyology:   2n= 42, variability in the range between 63-68 chromosomes and 36%

of cells have more of 84 chromosomes, modal number of chromosomes 65-67,

 number of markers - 22 (differential dye)  

Plating efficiency:    60% (SPBIC)

Tumorigenicity:  tumorigenic in syngeneic animals

Other properties:

inducible tyrosine aminotrasferase.

Applications:  tumorigenicity, enzymology, cytotoxicity, cell biology.

Collections: ICLC ATL 95006; SPBIC.