HaK

Origin: Syrian hamster, kidney

            S.Afr.J.Med.Sci. 1963. 28: 81.

Morphology:  epithelial-like

Mode of cultivation: monolayer

Conditions for cultivation: medium - BME

serum -   FBS 10%

subculture procedure - cells detach from flask using trypsin 0.25%:

EDTA  0.02% (1:1), split ratio 1:5 - 1:6, optimal population density  2.0-4.0104 cells/cm2

cryoconservation - growth medium,  10% DMSO, 1.0x106 cells/ml in ampule

Viability after cryoconservation:     86% (0 passage, dye trypan blue)

Sterility: tests for bacteria, fungi and mycoplasma were negative

Species: karyological and isoenzymological (LDH, G6PD) analysis

Karyology:   2n= 44, variability in the range between 48-58 chromosomes,

modal number of chromosomes 52-53 and 56, number of polyploid cells 5%.

Plating efficiency:    50%  (ATCC)

Tumorigenicity:  tumorigenic in hamster

Other properties:

virus susceptibility: vesicular stomatitis, arboviruses, Coxsackie A4, A8, B1,

herpes simplex, smallpox, Asian strain influenza, influenza, alpha viruses.

Applications:  virology.

Collections: ATCC CCL 15; ECACC 90102522; MWIIW; SPBIC.