GH3

Origin: rat, pituitary tumor.

            Endocrinology 1968. 82: 342; J.Cell Biol. 1969. 43: 432; In Vitro 1970. 60: 180.

Morphology:  epithelial-like

Mode of cultivation: monolayer

Conditions for cultivation: medium - F10

serum -   HS 15%, FBS 2.5%

subculture procedure - cells detach from flask using trypsin 0.25%:

EDTA  0.02% (1:3), split ratio 1:3 - 1:4, optimal population density  2.0-4.0104 cells/cm2

cryoconservation - growth medium, 10% DMSO, 1.0x106 cells/ml in ampule

Viability after cryoconservation:    85% (0 passage, dye trypan blue)

Sterility: tests for bacteria, fungi and mycoplasma were negative

Species: karyological analysis

Karyology:   2n= 42, variability in the range between 40-75 chromosomes

without modal number, number of markers - 2 dicentrics (routine dye), number of polyploid cells 0.6%.

Plating efficiency:    less than 1% (ATCC)

Tumorigenicity:  tumorigenic in syngeneic animals

Other properties:

virus susceptibility: vesicular stomatitis (Indiana), herpes simplex.

Growth hormone, prolactin, somatotrophin secretion.

Applications:  endocrinology, cell biology.

Collections: ATCC CCL 82.1; ECACC 87012603; ICLC ATL 96003; SPBIC.