Inf.Bull. Cell Culture Ass. St.-Petersburg, 1997, N12.
Mode of cultivation:
Conditions for cultivation:
medium - BME or 199
serum - FBS 5-10%
subculture procedure - cells detach from flask using trypsin 0.25%:
EDTA 0.02% (1:1), split ratio 1:5-1:20.
cryoconservation -BME 80%, FBS 10%, DMSO 10%, 2.0-3.0õ106 cells/ml in ampule
Viability after cryoconservation:
75-95% (0 passage, dye trypan blue)
tests for bacteria, fungi and mycoplasma were negative
karyological and/or isoenzymological (LDH and G6PD) analysis
Karyology: 2n= 38, variability in the range between 56-68 chromosomes,
modal number of chromosomes 62.
virus susceptibility: parvoviruses of carnivorous.