FLK

Origin: sheep, embryo, kidney.

            J.Natl.Cancer Inst. 1974. 52: 491; Bibl. Haemotol. 1976. 43: 360;

Canad. J. Comp. Med. 1981. 45: 154; Virology 1982. 122: 353;

J. Virol. Meth. 1983. 6: 19; Vopr. Virusol. (Russ.) 1983. 5: 615.

Morphology: epithelial-like

Mode of cultivation: monolayer

Conditions for cultivation: medium - BME

serum -   FBS 10%

subculture procedure - cells detach from flask using EDTA 0.02%

with 0.1 mg/ml chimopsin, split ratio 1:2-1:3, optimal population density 0.8-1.2105 cells/ml

cryoconservation - BME 80%, FBS 10%, glycerol 10%, 3.0-5.0106 cells/ml in ampule

Viability after cryoconservation:     90% (0 passage, dye trypan blue)

Sterility: tests for bacteria, fungi were negative

Species: karyological and/or isoenzymological (LDH, G6PD) analysis

Karyology:   2n= 54, variability in the range between 44-55 chromosomes,

modal number of chromosomes 54, pseudodiploid, there is one microchromosome, number of polyploid cells 4.0%.

Other properties:

Cells are BLV carriers and producers.

Applications: virology, biotechnology.

Collections: MWIIW; MWIEV.