FK-91

Origin: cat, kidney.

Morphology: epithelial-like

Mode of cultivation: monolayer

Conditions for cultivation: medium - EMEM

serum -   FBS 5-7%

subculture procedure - cells detach from flask using trypsin 0.25%:

EDTA  0.02% (1:9), split ratio 1:4-1:8.

cryoconservation -EMEM 60%, FBS 30%, DMSO or glycerol 10%, 2.0-3.0106 cells/ml in ampule

Viability after cryoconservation:     80% (0 passage, dye trypan blue)

Sterility: tests for bacteria, fungi and mycoplasma were negative

Species: karyological analysis

Karyology:   2n= 38, variability in the range between 56-67 chromosomes, modal number of chromosomes 64.

Other properties:

virus susceptibility: mink enteritis parvovitus.

Applications: virology, biotechnology.

Collections: MWIEV