Origin: bovine, embryo, trachea.

            Folia Biol. 1975. 21: 117.

Morphology:  epithelial-like

Mode of cultivation: monolayer

Conditions for cultivation: medium - DMEM

serum -   FBS 10%

subculture procedure - cells detach from flask using trypsin 0.25%:

EDTA  0.02% (1:3), split ratio 1:5.

cryoconservation - growth medium, 8-10% DMSO, 1.0x106 cells/ml in ampule

Viability after cryoconservation:     98% (0 passage, dye trypan blue)

Sterility: tests for bacteria, fungi and mycoplasma were negative

Species: karyological and isoenzymological (LDH, G6PD) analysis

Karyology:   2n= 60, variability in the range between 42-53 chromosomes,

modal number of chromosomes 48-49, number of polyploid cells 0.2%.

Other properties:

virus susceptibility: vesicular stomatitis, IBR, parainfluenza 3.

Applications:  virology.

Collections: MWIIW; SPBIC.