F-81

Origin: feline, kidney.

Morphology: fibroblast-like

Mode of cultivation: monolayer

Conditions for cultivation: medium - DMEM

serum -   FBS 10%

subculture procedure - cells detach from flask using using trypsin 0.25%:

EDTA  0.02% (1:9), split ratio 1:3

cryoconservation - DMEM 60%, FBS 30%, DMSO 10%, 5.0106 cells/ml in ampule

Viability after cryoconservation:     76% (0 passage, dye trypan blue)

Sterility: tests for bacteria, fungi were negative

Species: karyological analysis

Other properties:

virus susceptibility: BLV.

Applications: virology, cell biology.

Collections: MWIEV.