ESb1

Origin: cattle, embryo, 8-days blastocyst.

            Inf.Bull. Cell Culture Ass. St.-Petersburg 1997, N12.

Morphology:  cell clusters group

Mode of cultivation: on the feeder layer of mitotic inactivated mouse fibroblasts.

Conditions for cultivation: medium - a DMEM

serum -   NBS 10%

other components -   2-mercaptoethanol 0.1 mM, glucose 3.5 g/l, glutamine 2mM.

subculture procedure - mechanic method of cells detachment

with further colonies trypsinization

cryoconservation - a DMEM 70%, FBS 20%, glycerole 5%,

DMSO 10%, 2.0-3.0106 cells/ml in ampule

Viability after cryoconservation:     30-40% (0 passage, dye trypan blue)

Sterility: tests for bacteria, fungi and mycoplasma were negative

Species: karyological analysis

Karyology:   2n= 60, modal number of chromosomes 58.

Applications: cell biology.

Collections: MWIEV