mouse C57Bl, glioblastoma induced by dimethylbenzanthracene and than passed in outbred mice.
Cytology, Russ. 1977. 19. 1: 95-100.
Mode of cultivation:
Conditions for cultivation:
medium - BME
serum - BS 10%
subculture procedure - cells detach from flask using trypsin 0.25%: EDTA 0.02% (1:1), split ratio 1:5
cryoconservation - growth medium, 10% DMSO, 1.0-2.0õ106 cells/ml in ampule
Viability after cryoconservation:
94% (0 passage, dye trypan blue)
tests for bacteria, fungi and mycoplasma were negative
karyological, isoenzymological (LDH, G6PD) and immunofluorescent analysis
2n= 40, variability in the range between 57-64 chromosomes, modal number of chromosomes 59-60, number of markers-3-5 large-sized
metacentric and 1 middle acrocentric with secondary constriction ( routine dye), number of polyploid cells 1.5%.
tumorigenic in outbred mice
muscarinic and nicotinic receptors for acetylcholine and receptors for diazepam.
neurooncology, cell biology.