EPNT-5

Origin: mouse C57Bl, glioblastoma induced by dimethylbenzanthracene and than passed in outbred mice.

            Cytology, Russ. 1977. 19. 1: 95-100.

Morphology:  fibroblast-like

Mode of cultivation: monolayer

Conditions for cultivation: medium - BME

serum -   BS 10%

subculture procedure - cells detach from flask using trypsin 0.25%: EDTA  0.02% (1:1), split ratio 1:5

cryoconservation - growth medium, 10% DMSO, 1.0-2.0106 cells/ml in ampule

Viability after cryoconservation:     94% (0 passage, dye trypan blue)

Sterility: tests for bacteria, fungi and mycoplasma were negative

Species: karyological, isoenzymological (LDH, G6PD) and immunofluorescent analysis

Karyology:   2n= 40, variability in the range between 57-64 chromosomes, modal number of chromosomes 59-60, number of markers-3-5 large-sized metacentric and 1 middle acrocentric with secondary constriction ( routine dye), number of polyploid cells 1.5%.

Plating efficiency:    60%

Tumorigenicity:  tumorigenic in outbred mice

Other properties:

muscarinic and nicotinic receptors for acetylcholine and receptors for diazepam.

Applications:  neurooncology, cell biology.

Collections: SPBIC.