EL-4

Origin: mouse C57BL/6N, lymphoma induced by dimethyl-benzanthracene (ascitic fluid).

            Br.J.Cancer 1950. 4:372; Cancer Res. 1965. 25: 813;

J.Natl.Cancer Inst. 1972. 48: 265; J.Jmmunol. 1972. 108:1146; J.Jmmunol.1973. 110: 1470.

Morphology:  lymphoid

Mode of cultivation: suspension

Conditions for cultivation: medium - RPMI 1640

serum -   FBS 10%

subculture procedure - optimal population density 3.0-9.0x105  cells/ml

cryoconservation - growth medium, 10% DMSO, 5.0-6.0106 cells/ml in ampule

Viability after cryoconservation:     70% (0 passage, dye trypan blue)

Sterility: tests for bacteria, fungi and mycoplasma were negative

Species: karyological and isoenzymological (LDH, G6PD) analysis

Karyology:   2n= 40, variability in the range between 36-40 chromosomes,

modal number of chromosomes 38 and 40, number of markers - 3-4 (routine dye),

number of polyploid cells 2.2%.

Other properties:

antigens expressed by these cells include: G, a surface antigen induced by

leukemia type G virus; H-2b and Thy-1,2.

These cells do not bear TL antigen or surface immunoglobulin.

Resistant to cortisol and dexamethasone.

Sensitive to PHA.

Applications:  virology, tumorigenicity, biotechnology (IL-2 and interferon production).

Collections: ATCC TIB 39; ECACC 85022105; SPBIC.