mouse C57BL/6N, lymphoma induced by dimethyl-benzanthracene (ascitic fluid).
Br.J.Cancer 1950. 4:372; Cancer Res. 1965. 25: 813;
J.Natl.Cancer Inst. 1972. 48: 265; J.Jmmunol. 1972. 108:1146; J.Jmmunol.1973. 110:
Mode of cultivation:
Conditions for cultivation:
medium - RPMI 1640
serum - FBS 10%
optimal population density
cryoconservation - growth medium, 10% DMSO,
5.0-6.0õ106 cells/ml in ampule
Viability after cryoconservation:
70% (0 passage, dye trypan blue)
tests for bacteria, fungi and mycoplasma were negative
karyological and isoenzymological (LDH, G6PD) analysis
Karyology: 2n= 40, variability in the range between 36-40 chromosomes,
modal number of chromosomes 38 and 40, number of markers - 3-4 (routine dye),
number of polyploid cells 2.2%.
antigens expressed by these cells include: G, a surface antigen induced by
leukemia type G virus; H-2b and Thy-1,2.
These cells do not bear TL antigen or surface immunoglobulin.
Resistant to cortisol and dexamethasone.
Sensitive to PHA.
virology, tumorigenicity, biotechnology (IL-2 and interferon production).
Collections: ATCC TIB 39; ECACC 85022105; SPBIC.