Mode of cultivation:
Conditions for cultivation:
medium - EMEM or EMPH-d
serum - FBS 10%
subculture procedure - cells detach from flask using trypsin 0.25%:
EDTA 0.02% (1:9), split ratio 1:2-1:4
cryoconservation - culture medium 50%, FBS 40%, DMSO 10%, 2.0-3.0õ106 cells/ml in ampule
Viability after cryoconservation:
80-90% (0 passage, dye trypan blue)
tests for bacteria, fungi and mycoplasma were negative
Karyology: 2n= 64, variability in the range between 36-95 chromosomes,
modal number of chromosomes 40-50 and 80-90.
virus susceptibility: infectious anaemia of equine, herpesvirus of equine 2.