EKL

Origin: equine, skin.

Morphology: epithelial-like

Mode of cultivation: monolayer

Conditions for cultivation: medium - EMEM or EMPH-d

serum -   FBS 10%

subculture procedure - cells detach from flask using trypsin 0.25%:

 EDTA  0.02% (1:9), split ratio 1:2-1:4

cryoconservation - culture medium 50%, FBS 40%, DMSO 10%, 2.0-3.0106 cells/ml in ampule

Viability after cryoconservation:     80-90% (0 passage, dye trypan blue)

Sterility: tests for bacteria, fungi and mycoplasma were negative

Species: karyological analysis

Karyology:   2n= 64, variability in the range between 36-95 chromosomes,

modal number of chromosomes 40-50 and 80-90.

Other properties:

virus susceptibility: infectious anaemia of equine, herpesvirus of equine 2.

Applications: virology, biotechnology.

Collections: MWIEV