EHT

Origin: Syrian hamster, spontaneously transformed fibroblasts.

Ann.Microbiol.(Paris).1978. 129A: 379; J.Natl.Cancer Inst.1979. 62: 129.

Morphology:  epithelial- and fibroblast-like

Mode of cultivation: monolayer

Conditions for cultivation: medium - BME

serum -   FBS 10%

subculture procedure - cells detach from flask using EDTA 0.02%

with 0.1 mg/ml chimopsin, split ratio 1:3-1:5, optimal population density 1.0x105 cells/cm2

cryoconservation - BME 70%, FBS 20%, glycerol 10%, 3.0-5.0106 cells/ml in ampule

Viability after cryoconservation:     90% (0 passage, dye trypan blue)

Sterility: tests for bacteria, fungi and mycoplasma were negative

Species: karyological and isoenzymological (LDH, G6PD) analysis

Karyology:   2n= 44, variability in the range between 40-50 chromosomes,

modal number of chromosomes 46, number of markers - 1 large submetacentric

chromosome (routine dye), number of polyploid cells 2.0%.

Tumorigenicity:  non tumorigenic in nude mice

Other properties:

virus susceptibility: herpes viruses.

Applications: virology.

Collections: MWIIW.