Syrian hamster, fibroblasts, transformed by herpes simplex 1.
Ann.Microbiol.(Paris).1978. 129A: 379; J.Natl.Cancer Inst.1979. 62: 129.
Mode of cultivation:
Conditions for cultivation:
medium - BME
serum - FBS 10%
subculture procedure - cells detach from flask using EDTA 0.02%
with 0.1 mg/ml chimopsin, split ratio 1:3, optimal population density 1.0x105 cells/ml
cryoconservation - BME 70%, FBS 20%, glycerol 10%, 3.0-5.0õ106 cells/ml in ampule
Viability after cryoconservation:
98-99% (0 passage, dye trypan blue)
tests for bacteria, fungi and mycoplasma were negative
karyological and isoenzymological (LDH, G6PD) analysis
Karyology: 2n= 44, variability in the range between 39-57 chromosomes,
modal number of chromosomes 55, number of markers - 1, large metacentric
chromosome (routine dye), one
microchromosome is present, number of polyploid cells 2.0%.
high tumorigenic in nude mice and newborn hamsters.
virus susceptibility: herpes viruses.
Synthesis of virusspecific iRNA, but virusspecific antigens of HSV are not discovered.
Infectivity of herpes virus DNA is higher at 2-20 times, than in control cells EHT.
Cells are detected spontaneuos degeneration.