EH/A44

Origin: Syrian hamster, fibroblasts, transformed by herpes simplex 1.

Ann.Microbiol.(Paris).1978. 129A: 379; J.Natl.Cancer Inst.1979. 62: 129.

Morphology: epithelial-like

Mode of cultivation: monolayer

Conditions for cultivation: medium - BME

serum -   FBS 10%

subculture procedure - cells detach from flask using EDTA 0.02%

with 0.1 mg/ml chimopsin, split ratio 1:3, optimal population density 1.0x105 cells/ml

cryoconservation - BME 70%, FBS 20%, glycerol 10%, 3.0-5.0106 cells/ml in ampule

Viability after cryoconservation:     98-99% (0 passage, dye trypan blue)

Sterility: tests for bacteria, fungi and mycoplasma were negative

Species: karyological and isoenzymological (LDH, G6PD) analysis

Karyology:   2n= 44, variability in the range between 39-57 chromosomes,

modal number of chromosomes 55, number of markers - 1, large metacentric

chromosome (routine dye), one microchromosome is present, number of polyploid cells 2.0%.

Tumorigenicity:  high tumorigenic in nude mice and newborn hamsters.

Other properties:

virus susceptibility: herpes viruses.

Synthesis of virusspecific iRNA, but virusspecific antigens of HSV are not discovered.

Infectivity of herpes virus DNA is higher at 2-20 times, than in control cells EHT.

Cells are detected spontaneuos degeneration.

Applications: virology.

Collections: MWIIW.