Chinese hamster, ovary, clone of CHO.
Submitted from Columbia University,
New York, USA, 1984.
Mode of cultivation:
Conditions for cultivation:
medium - F12
serum - FBS 10%
subculture procedure - cells detach from flask using trypsin 0.25%:
EDTA 0.02% (1:3), split ratio 1:3
cryoconservation - growth medium, 10% DMSO,
1.0-2.0õ106 cells/ml in ampule
Viability after cryoconservation:
90% (0 passage, dye trypan blue)
tests for bacteria, fungi and mycoplasma were negative
Karyology: 2n= 22, variability in the range between 18-22 chromosomes,
modal number of chromosomes 20-21, number of markers - 2 large metacentric
(routine dye), number of polyploid cells 1.2%.
dihydrofolate reductase deficient, requires hypoxanthine or adenine, glycine, thymidine and proline.
biochemistry, cell biology.