Origin: Chinese hamster, ovary, clone of CHO.

            Submitted from Columbia  University, New York, USA, 1984.

Morphology:  epithelial-like

Mode of cultivation: monolayer

Conditions for cultivation: medium - F12

serum -   FBS 10%

subculture procedure - cells detach from flask using trypsin 0.25%:

EDTA  0.02% (1:3), split ratio 1:3

cryoconservation - growth medium, 10% DMSO, 1.0-2.0106 cells/ml in ampule

Viability after cryoconservation:     90% (0 passage, dye trypan blue)

Sterility: tests for bacteria, fungi and mycoplasma were negative

Species: karyological analysis

Karyology:   2n= 22, variability in the range between 18-22 chromosomes,

modal number of chromosomes 20-21, number of markers - 2  large metacentric

chromosomes (routine dye), number of polyploid cells 1.2%.

Other properties:

dihydrofolate reductase deficient, requires hypoxanthine or adenine, glycine, thymidine and proline.

Applications:  biochemistry, cell biology.

Collections: SPBIC