DSHCHS

Origin: pig, thyroid.

            Trudy of VIEV. Moscow. 1987. 64: 34-35.

Morphology: epithelial-like

Mode of cultivation: monolayer

Conditions for cultivation: medium - 199

serum -   FBS 10%

other components - 

subculture procedure - cells detach from flask using trypsin 0.25%:

EDTA  0.02% (1:9), split ratio 1:2-1:3

cryoconservation -  medium 199 50%, FBS 40%, DMSO 10%, 2.0-3.0106 cells/ml in ampule

Viability after cryoconservation:     75% (0 passage, dye trypan blue)

Sterility: tests for bacteria, fungi and mycoplasma were negative

Species: karyological analysis

Karyology:   2n=38, variability in the range between 36-57 chromosomes,

modal number of chromosomes 38.

Other properties:

production of thyroid hormones (3-iodothyronine and thyroxin)

Applications: virology, biotechnology.

Collections: MWIEV.