DLEK

Origin: cattle, embryo, lung.

            USSR Patent N 1306121, 1986.

Morphology: epithelial-like

Mode of cultivation: monolayer

Conditions for cultivation: medium - BME

serum -   BS 10%

subculture procedure - cells detach from flask using trypsin 0.25%:

EDTA  0.02% (1:9), split ratio 1:2-1:3

cryoconservation - growth medium 45%, FBS 45%, 10% DMSO, 3.0-4.0106 cells/ml in ampule

Viability after cryoconservation:     80% (0 passage, dye trypan blue)

Sterility: tests for bacteria, fungi were negative

Species: karyological analysis

Karyology:   2n= 60.

Other properties:

virus susceptibility: equine rhinopneumonia; infectious bovine rhinotracheitis;

parainfluenza 3; calf rotavirus.

Applications: virology, biotechnology.

Collections: MWIEV.