DKMEKr-85

Origin: rabbit, skin-muscle tissue.

            Inf.Bull. Cell Culture Ass. St.-Petersburg, 1997, N12.

Morphology: fibroblast-like

Mode of cultivation: monolayer

Conditions for cultivation: medium -  0.5% LAH/EMEM (1:1)

serum -   BS 5-12%

subculture procedure - cells detach from flask using trypsin 0.25%:

EDTA  0.02% (1:5), split ratio 1:2.

cryoconservation - culture medium 70%, BS 20%, DMSO 10%, 2.0-3.0106 cells/ml in ampule

Viability after cryoconservation:     50-60% (0 passage, dye trypan blue)

Sterility: tests for bacteria, fungi and mycoplasma were negative

Species: isoenzymological (LDH, G6PD) analysis.

Karyology:   2n= 44, modal number of chromosomes 43-44, number of markers -1,

large submetacentric chromosome with deletion of one arm.

Plating efficiency:    60%

Other properties:

virus susceptibility: equine rhinopneumonia and arthritis; reovirus 3;

infectious rhinotracheitis; parainfluenza 3; bovine diarrhea.

Applications: virology.

Collections: MWIEV