DFK3

Origin: rat, embryo, fibroblasts transformed by adenovirus 5.

            Mol.Biol. (Russ.) 1979. 13: 292.

Morphology:  fibroblast-like

Mode of cultivation: monolayer

Conditions for cultivation: medium - EMEM or DMEM

serum -  FBS 10%

subculture procedure - cells detach from flask using EDTA 0.02%, split ratio 1:3

cryoconservation - growth medium, 10% DMSO, 2.0106 cells/ml in ampule

Viability after cryoconservation:     85% (0 passage, dye trypan blue)

Sterility: tests for bacteria, fungi and mycoplasma were negative

Species: karyological and isoenzymological (LDH, G6PD) analysis

Karyology:   2n=42, variability in the range between 55-67 chromosomes,

modal number of chromosomes 64-65, number of polyploid cells 3.0%.

Applications:  cell biology.

Collections: SPBIC