D5x5

Origin: hybrid cells - pig kidney SPEV TK- x bovine lymphocytes.

Submitted by Sh.M.Tugisov, 1984; Tugizov Sh.M., PhD Thesis, M.,1985.

Morphology: epithelial- and lymphocyte-like

Mode of cultivation: monolayer

Conditions for cultivation: medium - MEM

serum - FBS 10%

subculture procedure - cells detach from flask using trypsin 0,25% : EDTA 0,02% (1:10), split ratio 1:4-1:5

cryoconservation - EMEM 50%, FBS 40%, DMSO 10%, 2-3x10 6 cells/ml in ampule

Viability after cryoconservation: 75% (0 passage, dye trypan blue)

Sterility: tests for bacteria,fungi and mycoplasma were negative

Other properties:

production of BLV

Applications: cell biology, virology.

Collections: MWIEV