Origin: hybrid cells - pig kidney SPEV TK- x bovine lymphocytes.
Submitted by Sh.M.Tugisov, 1984; Tugizov Sh.M., PhD Thesis, M.,1985.
Morphology: epithelial- and lymphocyte-like
Mode of cultivation: monolayer
Conditions for cultivation: medium - MEM
serum - FBS 10%
subculture procedure - cells detach from flask using trypsin 0,25% : EDTA 0,02% (1:10), split ratio 1:4-1:5
cryoconservation - EMEM 50%, FBS 40%, DMSO 10%, 2-3x10 6 cells/ml in ampule
Viability after cryoconservation: 75% (0 passage, dye trypan blue)
Sterility: tests for bacteria,fungi and mycoplasma were negative
production of BLV
Applications: cell biology, virology.