CV-1

Origin: African green monkey, kidney.

            Proc.Natl.Acad.Sci. 1964. 53: 53; Virology 1965. 27: 453.

Morphology:  fibroblast-like

Mode of cultivation: monolayer

Conditions for cultivation: medium - EMEM or DMEM

serum -   FBS 10%

other components -   NEAA 1% (EMEM)

subculture procedure - cells detach from flask using trypsin 0.25%:

EDTA  0.02% (1:3), split ratio 1:3 - 1:6, optimal population density 2.0-4.0104 cells/cm2

cryoconservation - growth medium, 5%DMSO, 1.0106 cells/ml in ampule

Viability after cryoconservation:     85% (0 passage, dye trypan blue)

Sterility: tests for bacteria, fungi and mycoplasma were negative

Species: karyological and isoenzymological (LDH, G6PD) analysis

Karyology:   2n= 60, variability in the range between 56-61 chromosomes,

modal number of chromosomes 60, number of markers - 4-5 (differential dye),

number of polyploid cells 4.4% (ATCC).

Plating efficiency:    27% (ATCC)

Other properties:

virus susceptibility: poliovirus 1, herpes simplex, Eastern equine encephalitis,

Western equine encephalitis, California encephalitis, SV 40.

Applications:  virology.

Collections: ATCC CCL 70; ECACC 87032605; SPBII; MWIIW; SPBIC.