CRFK

Origin: feline, kidney.

            J. Amer. Vet. Med. Assoc. 1971. 158: 976; J.Natl.Cancer Inst. 1972. 41: 55; In Vitro 1973. 9: 176.

Morphology: epithelial-like

Mode of cultivation: monolayer

Conditions for cultivation: medium - DMEM

serum -    FBS 15%

other components -   glucose 4 g/l, glutamine 2mM, sodium pyruvate 1mM.

subculture procedure - cells detach from flask using EDTA 0.02%

with 0.1 mg/ml chimopsin, split ratio 1:4-1:8, optimal population density 0.5-0.7105 cells/ml

cryoconservation - DMEM 70%, FBS 20%, glycerol 10%, 2.0-5.0106 cells/ml in ampule

Viability after cryoconservation:     89% (0 passage, dye trypan blue)

Sterility: tests for bacteria, fungi and mycoplasma were negative

Species: karyological and isoenzymological (LDH, G6PD) analysis

Karyology:   2n= 38, variability in the range between 32-38 chromosomes,

modal number of chromosomes 36, number of markers - 1 large submetacentric

chromosome (routine dye), number of polyploid cells 4.0%.

Tumorigenicity:  non tumorigenic

Other properties:

virus susceptibility: feline calicivirus, herpes, picornaviruses, rhynotracheitis, reovirus, canine parvoviruses, rabies.

Applications: virology.

Collections: ATCC CCL 94; ECACC 86093002; MWIIW.