Chinese hamster, ovary, subline of CHO-K1.
Proc.Natl.Acad.Sci. 1969. 64: 1284; Science 1969. 164. 312.
Mode of cultivation:
Conditions for cultivation:
medium - BME
serum - FBS 10%
subculture procedure - cells detach from flask using EDTA 0.02%
with 0.1 mg/ml chimopsin, split ratio 1:3-1:5, optimal population density 1.0õ105
cryoconservation - BME 70%, FBS 20%, glycerol 10%, 4.0-5.0õ106 cells/ml in ampule
Viability after cryoconservation:
92-97% (0 passage, dye trypan blue)
tests for bacteria, fungi and mycoplasma were negative
karyological and isoenzymological (LDH, G6PD) analysis
Karyology: 2n= 22, variability in the range between 10-27 chromosomes,
modal number of chromosomes 22, pseudodiplid, there are microchromosomes, number of polyploid cells 4.0%.
virus susceptibility: vesicular stomatitis, arboviruses.
The cells have deficiency in synthesis proline and interferon.