CHO-K1v

Origin: Chinese hamster, ovary, subline of CHO-K1.

Proc.Natl.Acad.Sci. 1969. 64: 1284; Science 1969. 164. 312.

Morphology: epithelial-like

Mode of cultivation: monolayer

Conditions for cultivation: medium -  BME

serum -   FBS 10%

subculture procedure - cells detach from flask using EDTA 0.02%

with 0.1 mg/ml chimopsin, split ratio 1:3-1:5, optimal population density  1.0105 cells/ml

cryoconservation - BME 70%, FBS 20%, glycerol 10%, 4.0-5.0106 cells/ml in ampule

Viability after cryoconservation:     92-97% (0 passage, dye trypan blue)

Sterility: tests for bacteria, fungi and mycoplasma were negative

Species: karyological and isoenzymological (LDH, G6PD) analysis

Karyology:   2n= 22, variability in the range between 10-27 chromosomes,

modal number of chromosomes 22, pseudodiplid, there are microchromosomes, number of polyploid cells 4.0%.

Other properties:

virus susceptibility: vesicular stomatitis, arboviruses.

The cells have deficiency in synthesis proline and interferon.

Applications: virology.

Collections: MWIIW.