CHO-K1

Origin: Chinese hamster, ovary, clone CHO.

            J.Exp.Med. 1958. 108: 945; Proc. Natl.Acad.Sci. USA 1968. 60: 1275.

Morphology:   epithelial-like

Mode of cultivation: monolayer

Conditions for cultivation: medium - F12

serum -   FBS 10%

subculture procedure - cells detach from flask using trypsin 0.25%:

EDTA  0.02% (1:1), split ratio 1:4 - 1:15, optimal population density 1.0-2.0104 cells/cm2

cryoconservation - growth medium, 10% DMSO, 1.0-2.0106 cells/ml in ampule

Viability after cryoconservation:     99% (0 passage, dye trypan blue)

Sterility: tests for bacteria, fungi and mycoplasma were negative

Species: karyological, isoenzymological (LDH, G6PD)  and immunofluorescent analysis

Karyology:   2n=22, variability in the range between 16-22 chromosomes,

modal number of chromosomes 20, number of markers - 12 (differential dye).

Plating efficiency:    90% (ATCC)

Other properties:

virus susceptibility: vesicular stomatitis (Indiana), Getah arbovirus.

Absence of the gene for proline synthesis, requirement of proline for growth.

Applications:  somatic cells genetics, cell biology, virology.

Collections: ATCC CCL 61; ECACC 85051005; DSM ACC 110; ESCC; SPBIC.