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Origin: feline, embryo, fibroblasts transformed by Moloney murine sarcoma virus.

            J.Virol. 1973. 11: 978; 1974. 14; 177.

Morphology: epithelial-like

Mode of cultivation: monolayer

Conditions for cultivation: medium - DMEM

serum -   FBS 15%

other components -   glucose 4 g/l, glutamine 2 mM, sodium pyruvate 1mM.

subculture procedure - cells detach from flask using EDTA 0.02%

with 0.1 mg/ml chimopsin, split ratio 1:6-1:10, optimal population density 0.5-0.7x105 cells/ml

cryoconservation - DMEM 70%, FBS 20%, glycerol 10%, 2.0-3.0106 cells/ml in ampule

Viability after cryoconservation:     89% (0 passage, dye trypan blue)

Sterility: tests for bacteria, fungi and mycoplasma were negative

Species: karyological and isoenzymological (LDH and G6PD) analysis

Karyology:   2n= 38, variability in the range between 26-32 chromosomes,

modal number of chromosomes 30, number of markers -1, large submetacentric

chromosome (routine dye), number of polyploid cells 8.0%.

Other properties:

virus susceptibility: vesicular stomatitis; feline leukemia; bovine syncytium-forming virus.

Presence of murine sarcoma virus genome.

Production of oncornaviruses RD-114 and murine sarcoma. 

Applications: virology.

Collections:  MWIIW