C6

Origin: rat, glioma induced in vivo by N-nitrosomethylurea, monoclonal cell line.

            Science 1968. 161: 370; Fed.Proc. 1968. 27: 720.

Morphology:  fibroblast-like

Mode of cultivation: monolayer

Conditions for cultivation: medium - F10 or F12

serum -   FBS 10% (F12) or HS 15%/FBS 2.5% (F10)

subculture procedure - cells detach from flask using trypsin 0.25%:

EDTA  0.02% (1:3), split ratio 1:3 - 1:6, optimal population density 1.0-3.0105 cells/cm2

cryoconservation - growth medium, 5%DMSO, 2.0-3.0106 cells/ml in ampule

Viability after cryoconservation:     93% (0 passage, dye trypan blue)

Sterility: tests for bacteria, fungi and mycoplasma were negative

Species: karyological, immunofluorescent and isoenzymological (LDH, G6PD) analysis

Karyology:   2n= 42, variability in the range between 39-44 chromosomes,

modal number of chromosomes 42, normal rat karyotype (42, XY),

cells containing  43 chromosomes have 1 marker (differential dye).

Plating efficiency:    26% (SPBIC)

Tumorigenicity:  tumorigenic in albino rat

Other properties:

virus susceptibility: pseudorabies, vesicular stomatitis (Indiana), herpes simplex, vaccinia.

S 100 protein production

Applications:  biochemistry, virology, differentiation, tumorigenicity.

Collections: ATCC CCL 107; ECACC 85040101; ICLC ATL 95007; SPBIC.