C2C12

Origin: mouse C3H, leg muscle.

            Nature 1977. 270: 725-727; Science 1985. 230: 758-766.

Morphology:  myoblast-like

Mode of cultivation: monolayer

Conditions for cultivation: medium - DMEM

serum -   FBS 10%

subculture procedure - cells detach from flask using trypsin 0.25%:

EDTA  0.02% (1:3), split ratio 1:3 - 1:6, optimal population density 2.0-4.0104 cells/cm2

cryoconservation - growth medium, 10% DMSO, 1.0x106 cells/ml in ampule

Viability after cryoconservation:     90-95% (0 passage, dye trypan blue)

Sterility: tests for bacteria, fungi and mycoplasma were negative.

Species: karyological and isoenzymological (LDH, G6PD) analysis

Other properties:

muscle protein expression.

Differentiates producing myotubes.

Applications:  myogenesis, cell differentiation, cell biology.

Collections: ATCC CRL 1772; ECACC 91031101; SPBIC.